There were no significantly different(p>0.05) on the percentages of motile and viable sperm in SMT (21.7% and 43.4%, respectively) compared with those extended with DV T extender (26.9% and 50.8%, respectively), DMF demonstrated better results as CPA compared to the others and DVTDMF combination had the best protection during cryopreservation in this study. After 24 hours, the semen was thawed at 37вЊ for 30 second. The extended semen was individually packed in 0.3 ml minitube, equilibrated at 4вЊ for 2 hours, frozen in liquid nitrogen vapor for 10 minutes, and then was stored in liquid nitrogen container at -196вЊ. The supernatan discarded, the pellet was rediluted by SM trehalosa or DV trehalose, and added by G, EG+G ly, or D MF to reach the final sperm concentration of 200x 1 06/ml. 'Tube extension carries an extra 2 rounds of 12 gauge with a very slight weight increase.' Description when used on the Model 680 The Tube Extension is an attachment available to the Model 680 in Call of Duty: Modern Warfare.

After being diluted, all tubes were centrifuged at 1006xg for 10 minutes. It is a network of networks that consists of private, public, academic, business, and government networks of local to global scope, linked by a broad array of. Each semen sample was evaluated and divided equally into six tubes semen in the three tubes was diluted 1:1 with (SM), while in the remaining tubes the semen was diluted 1: 1 by DY. The Internet (or internet) a is the global system of interconnected computer networks that uses the Internet protocol suite (TCP/IP) b to communicate between networks and devices. Extender III containing 0.8 lecithin was from Minitube (Tiefenbach. Skim milk (SM) and Dimitropoulos (DV) were the extenders used in this study each was added by glycerol (Gly), combination of ethylene glycol-glycerol (EG+Gly) or dimethilformamide (OMF). TRIS-EY + Gly + Equex extender was superior regarding progressive motility. Continuous sets are used when feeding small amounts constantly throughout the day (or night) without interruption. The objective of this study was to assess different CPAs on stallion semen cryopreservation. The extension set attaches to any MIC-KEY feeding tube when nutrition, fluids, or medication needs to be given. Cryoprotective agents (CPAs) protect sperm during cryopreservation.

Minitube extension